Journal: ACS Omega

Article Title: Differential Glycosylation Expression in Injured Rat Spinal Cord Treated with Immunosuppressive Drug Cyclosporin-A

doi: 10.1021/acsomega.8b02524

Figure Lengend Snippet: Intensity of primary astrocyte and Neu7 cell protein extracts binding to lectins on microarray. Bar chart representing the differences in binding of fluorescently labeled protein extracts to printed lectins on a microarray surface where Ast_Lys is the total lysate from primary astrocytes, Ast_Cyt is the cytosolic (hydrophilic) protein-enriched extract from primary astrocytes, Ast_Mem is the membrane (hydrophobic) protein-enriched extract from primary astrocytes, Neu7_Lys is the total lysate from Neu7 cells, Neu7_Cyt is the cytosolic (hydrophilic) protein-enriched extract from Neu7 cells, and Neu7_Mem is the membrane (hydrophobic) protein-enriched extract from Neu7 cells. Graph represents the mean of three replicate experiments (except for duplicate experiments for Neu7_Mem binding to MAL-II), with each experiment the median of six individual replicates. Error bars represent ±1 standard deviation of the mean of the three experiments.

Article Snippet: The lectins were printed at approximately 1 nL per feature on Nexterion Slide H microarray slides using a sciFLEXARRAYER S3 piezoelectric printer (Scienion AG, Berlin, Germany) as previously described.

Techniques: Binding Assay, Microarray, Labeling, Standard Deviation